Project #1023

Overcoming Molecular Sample Processing Limitations: Quantitative PCR

$0
Completed
Principal Investigator
George
Di Giovanni
Research Manager
Dr. Daniel M. Woltering, Ph.D.
Contractor
American Water
Microbes & Pathogens
Treatment

Abstract

Molecular methods appear to be a promising way to rapidly detect pathogens in water. However, researchers need to identify and evaluate sample preparation techniques that are high-throughput and cost-effective, require minimum training for use, and are reproducible from user to user. This research covers important topics in molecular sampling: RNA and DNA extraction strategies, quantitative polymerase chain reaction, and fiber-optic biosensors. Published in 2006.

Originally funded as WERF project 00-HHE-2B.

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