Project #2818

Development of Molecular Reporters for Microcystis Activity and Toxicity

$345,025
Completed
Principal Investigator
Steven
Wilhelm
Research Manager
Michaela N Hasan
Contractor
University of Tennessee

Abstract

Studies novel molecular approaches for the detection of the toxic cyanobacterium Microcystis aeruginosa and the potent hepatotoxin it produces-microcystin. Combines polymerase chain reaction-based techniques with genomic analyses to establish specific genetic markers that can be used to identify M. aeruginosa or to diagnose the potential for in situ toxin production in cultured isolates, as well as in natural samples. Identifies genetic elements that can be incorporated into biosensors for subsequent real-time diagnostics in water treatment facilities. Completed in 2007. Foundation subscribers can download this Web-only report.