Project 2753

Comparing Cell Culture and Mouse Assays for Measuring Infectivity of Cryptosporidium

$428,746
Completed
Principal Investigator
Paul
Rochelle
Research Manager
Michaela N Hasan
Contractor
Metropolitan Water District of So California
Microbes & Pathogens
Monitoring

Abstract

Analyzes the infectivity of Cryptosporidium parvum oocysts by comparing several different cell cultural methods to animal infectivity models. Also compares the inactivation of oocysts by a variety of disinfectants using cell culture and animal infectivity models. Determines the deviation in results associated with animal infectivity assays to measure ozone inactivation of Cryptosporidium. Develops approaches to reduce statistical errors in determining ozone CT values. Provides new CT tables for ozone using robust data from a statistically designed and executed experimental plan that will minimize the standard deviation of results. Research partner: USEPA. Published in 2004.