Method(s) for Detecting and Differentiating Opportunistic Premise Plumbing Pathogens (OPPPs) to Determine Efficacy of Control and Treatment Technologies
During this first reporting period, the team held its first project advisory committee (PAC) meeting, the target opportunistic pathogens (OPs) were selected, and development of DNA extraction and quantitative polymerase chain reaction (qPCR) methods was initiated. The selected OPs are Mycobacterium avium, Pseudomonas aeruginosa, Legionella pneumophila, and Acanthamoeba. All strains being used for testing are environmentallyisolated and tested at the early stationary growth phase to best approximate distribution system and premise plumbing conditions.
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