Interlaboratory and Methods Assessment of the SARS-CoV-2 Genetic Signal in Wastewater


Currently, no standard methods have been established for the detection of the genetic signal for SARS-CoV-2 in wastewater. While surveillance of wastewater has the potential to serve as a useful gauge of community-level trends in SARS-CoV-2, the data will be most useful for public health decision makers if they can be demonstrated to be repeatable and comparable across laboratories and facilities that are analyzing samples.

This webcast presented the findings of the project, Interlaboratory and Methods Assessment of the SARS-CoV-2 Genetic Signal in Wastewater (5089). The goal of the study was to provide an assessment of 36 distinct analytical methods currently used at 32 U.S.-based laboratories and facilities to determine which method(s) provide reliable and repeatable measurement of the SARS-CoV-2 genetic signal (i.e., copies of RNA) in untreated wastewater. The project results provide much-needed information on preferred methods to use when performing laboratory analyses for sewer surveillance studies. The webcast also included an extended Q&A session. The study has been submitted for open access journal publication and the pre-print manuscript is available now. The project's executive summary and quality assurance plan (QAPP) are currently available on the WRF project page.

Stephanie Fevig, Research Program Manager, The Water Research Foundation

Peter Grevatt, PhD, Chief Executive Officer, The Water Research Foundation

Presentation of Results
Brian Pecson, PhD, PE, Principal Engineer, Trussell Technologies
Emily Darby, Trussell Technologies

Question and Answer Session
Christobel Ferguson, PhD, Chief Innovation Officer, The Water Research Foundation