Methods for Detecting and Differentiating Opportunistic Premise Plumbing Pathogens (OPPPs) to Determine Efficacy of Control and Treatment Technologies
The presence of opportunistic pathogens (OPs) in drinking water is a growing public health concern and a potential target for future regulations in the United States. DNA-based methods, such as quantitative polymerase chain reaction (qPCR), offer rapid and specific alternatives to culture-based methods. However, the variation in methods used has slowed adoption of qPCR in practice and limited comparability between studies.
Methods for Detecting and Differentiating Opportunistic Premise Plumbing Pathogens (OPPPs) to Determine Efficacy of Control and Treatment Technologies (4721) sought to develop optimized, validated qPCR methods for the quantification of four OPs (Legionella pneumophila, Pseudomonas aeruginosa, species of nontuberculous mycobacteria, and Acanthamoeba spp.) in drinking water. The selected methods were then validated for characterizing the impacts of OP control technologies in building water. Project products included a literature review on monitoring technologies, comparisons of DNA extraction and qPCR methods, a validation study for the qPCR methods, and guidance for drinking water utilities on methods for OP monitoring. This webcast focused on culture-independent drinking water OP quantification methods and laboratory experiments evaluating the efficacy of OP mitigation strategies in building plumbing using the selected qPCR methods.
Lutgarde Raskin, PhD, NAE, Vernon L. Snoeyink Distinguished University Professor of Environmental Engineering, Altarum/ERIM Russell O'Neal Professor of Engineering, Department of Civil and Environmental Engineering, University of Michigan
Katherine Dowdell, PhD, PE, Postdoctoral Researcher Civil, Architectural, and Environmental Engineering, The University of Texas at Austin
Marc A. Edwards, Charles Lunsford Professor, University Distinguished Professor, Department of Civil and Environmental Engineering, Virginia Tech
Grace Jang, PhD, Research Program Manager, The Water Research Foundation